In Hi-C, DNA loci that are spatially proximate in a system of interest (such as a cell nucleus or an in vitro DNA preparation) are ligated to one another and sequenced. Hi-C is a widely employed method for studying how genomes are folded in three dimensions (Rao et al., 2014). Maps can be compared to one another, or to 1D tracks or 2D feature sets. Juicebox allows users to zoom in and out of Hi-C maps interactively, just as a user of Google Earth might zoom in and out of a geographic map. Here we introduce Juicebox, a tool for exploring Hi-C and other contact map data. Hi-C experiments study how genomes fold in 3D, generating contact maps containing features as small as 20 bp and as large as 200 Mb.
Lander,4'5 6 and Erez Lieberman Aiden1'2'3'4'7 *ġThe Center for Genome Architecture, Baylor College of Medicine, Houston, TX 77030, USA 2Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USAģDepartment of Computer Science, Department of Computational and Applied Mathematics, Rice University, Houston, TX 77005, USA 4Broad Institute of Harvard and Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA 5Department of Biology, MIT, Cambridge, MA 02139, USAĦDepartment of Systems Biology, Harvard Medical School, Boston, MA 02115, USA 7Center for Theoretical Biological Physics, Rice University, Houston, TX 77030, USA 8Co-first author
Juicebox Provides a Visualization System for Hi-C Contact Maps with Unlimited Zoom
0 kommentar(er)
